The ability to detect AML-specific fusion genes is critical in both clinical and research settings.
The AML Q-Fusion Screening Kit is a novel, multiplex, reverse transcription real-time PCR (RT-qPCR) system able to simultaneously detect 16 fusion genes, including PML-RARα L and PML-RARα S. Fusion and control gene transcripts are co-amplified in each reaction and identified by specific fluorescent probes. The Q-Fusion system provides a sensitive, specific, reliable and cost-effective tool that is easy to perform for the routine screening of leukemia fusion transcripts.
For Research Use Only. Not intended for use in diagnostic procedures
|Fusion genes||MLL-AF9, CBFβ-MYH11, PML-RARα L, PML-RARα S, AML1-MDS1, NPM-MLF1, AML1-ETO, NPM-RARα, PLZF-RARα, DEK-CAN, MLL-ELL, AML1-EAP, MLL-AF10, SET-CAN, TEL-ABL1, TLS-ERG, NPM-ALK|
|Sample types||Bone marrow, peripheral blood, FFPE, and cell lines|
|Reaction Volume||25 ul|
|Reaction time||2-3 hour|
|Internal control||GUSB as housekeeping genes, 2 positive control, 3 negative control|
|Instruments||Compatible with Bio-Rad CFX96, iCycler, ABI7500, Stratagene Mx3000P/Mx3005P, and other commonly used RT-qPCR machines.|
AML Q-Fusion Screening Kit
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