Probe-Based Melting Curve Analysis


QuanDx’s HPV and TB test kit is based on the technology of 4-color melting curve analysis (MMCA). After PCR amplification, HPV or TB genotypes are identified by the different Tm values of hybridizing PCR products with the fluorescent probes. The hybridization probe that fully matches its wild-type target gives the highest Tm value. However, a mutant-type target paired with the probe gives a lower Tm value. Mutation is detected as Tm deviation (ΔTm) compared to the wild-type hybrid.

Courtesy to Zeesan Technology